FLUORESCENT COMPOUNDS. Blue-Emitting Mitochondrial Stain with High Photostability

A new environment-sensitive, blue-emitting fluorophore (N1,N3-di(2-aminidonaphthalen-6-yl)propane-1,3-diamine) that selectively stains functional mitochondria.

1. Long exposures maintaining emission intensity.
2. Compatible with available stains for multichannel experiments.
3. Used also in living cells.
4. Extreme resistance to photobleaching allows long exposures or multiple irradiation without loss of emission intensity.
5. Simple and easy to use.

Its emission wavelength in the blue channel (emission maximum at approximately 490 nm) is complementary to green and orange/red stains (Mitotracker dyes). In contrast with current stains, MitoBlue is extremely resistance to photobleaching, which allows longer or repeated exposures in long-term experiments.

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Compuestos fluorescentes capaces de teñir de forma selectiva las mitocondrias: MITOBLUE. La presente invención se dirige a los compuestos de formula I, a un kit que comprende dichos compuestos y a su aplicación como agentes de tinción. Dichos compuestos están diseñados para que sean capaces de internalizarse en la célula viva, acumularse selectivamente en las mitocondrias y teñirlas de forma preferente a otros orgánulos celulares. Además tienen una elevada fotoestabilidad. Adicionalmente, los compuestos de la invención permiten ser aplicados en cultivos celulares, tanto cultivos primarios como líneas celulares, bien en células vivas o bien fijadas con parafornaldehido, manteniendo la eficacia de la tinción y su selectividad, permitiendo su uso en experimentos de visualización en tiempo real.   The invention relates to the compounds of formula I, a kit comprising said compounds, and to the use of said compounds as selective markers for mitochondria. The compounds are designed such that they can be internalised by the living cell, selectively accumulate in the mitochondria, and stain said mitochrondria in preference to other cell organelles. The compounds also have an increased photostability. In addition, the compounds according to the invention can be used in cell cultures, both in primary cultures and cell lines, in living cells or paraformaldehyde-fixed living cells, with the selectivity and staining efficacy thereof being maintained, allowing same be used in real-time visualisation experiments.